Fig1: Western blot analysis of Prohibitin on different lysates using anti-Prohibitin antibody at 1/1,000 dilution. Positive control: Lane 1: 293 Lane 2: Jurkat Lane 3: Mouse kidney Lane 4: HepG2
Fig2: ICC staining Prohibitin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Prohibitin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.
Fig9: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.
Fig10: Flow cytometric analysis of HepG2 cells with Prohibitin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the seco antibody
Fig11:Western blot analysis of Prohibitin on hybrid fish (crucian-carp) liver tissue lysate using anti-Prohibitin antibody at 1/500 dilution.
Product Name | Prohibitin [SR46-02] |
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Antibody Type | Primary Antibodies |
Product description |
|
Immunogen | recombinant protein |
Clonality | Monoclonal |
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Isotype | IgG |
Host Species | Recombinant rabbit |
Tested Applications | |
WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:200-1:500 FC:1:50-1:100 |
|
Species Reactivity | |
Concentration | 1 mg/mL. |
Alternative Names | Epididymis luminal protein 215 antibody
Epididymis secretory sperm binding protein Li 54e antibody
HEL 215 antibody
HEL S 54e antibody
PHB antibody
PHB_HUMAN antibody
PHB1 antibody
Prohibitin antibody
|
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Molecular Weight(MW) | 30 kDa |
Cellular Localization | Mitochondrion inner membrane. |
SwissProt ID | P35232 P67778 P67779 |
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Fig1: Western blot analysis of Prohibitin on different lysates using anti-Prohibitin antibody at 1/1,000 dilution. Positive control: Lane 1: 293 Lane 2: Jurkat Lane 3: Mouse kidney Lane 4: HepG2
Fig2: ICC staining Prohibitin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Prohibitin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.
Fig9: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.
Fig10: Flow cytometric analysis of HepG2 cells with Prohibitin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the seco antibody
Fig11:Western blot analysis of Prohibitin on hybrid fish (crucian-carp) liver tissue lysate using anti-Prohibitin antibody at 1/500 dilution.
Positive Control | NIH/3T3, Jurkat, HepG2, human liver cancer tissue, mouse stomach tissue, human kidney tissue, mouse heart tissue, mouse liver tissue, mouse kidney tissue. |
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Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:200-1:500 FC:1:50-1:100 |
Form | Liquid |
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Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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